Figure 6.

Good responders upregulated DNA repair genes compared with poor responders upon Givinostat treatment and increased DNA damage protein pH2AX. (a) RAD50, MLH1, NBN, CDC73, JAG1 and DLL1 expression analysis by qRT-PCR in good (PD-TALL8, PD-TALL16, PD-TALL43) and poor responders (PD-TALL9 and PD-TALL6) after 6 h of treatment with Givinostat in vivo. PD-TALL8 (n=2 mice), PD-TALL16 (n=5 mice), PD-TALL43 (N=4 mice), PD-TALL9 (n=4 mice), PD-TALL6 (n=3 mice). The 2^{−Delta CT} (Delta CT=CT gene–CT Beta2 microglobulin) was used as a read out of quantitative RT-PCR data. (b) Cells were recovered from the spleen of the xenografts and treated in vitro with Givinostat or vehicle for 6 h. pH2AX and total H2AX protein levels were then analyzed by western blotting