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. 2016 Jan 28;7(1):e2074. doi: 10.1038/cddis.2015.402

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CDC27 is overexpressed in CRC cell lines and patient tissues and is associated with decreased survival in CRC. (a) Upregulation of CDC27 protein expression was detected by western blotting analysis in a normal colon epithelial cell line (FHC) and seven CRC cell lines. GAPDH was used as a reference control. (b) Representative images of CDC27 expression in CRC tissues and paired adjacent normal mucosal tissues by immunohistochemistry (× 200 magnification). (c) Relative immunohistochemstry analysis for CDC27 expression in CRC tissues and adjacent normal mucosal tissues; each point in the graph represents the CDC27 expression H-score of an individual patient tumor (n=166, ***P<0.001, paired Student's t-test). (d and e) Kaplan–Meier survival analysis of the association between CDC27 expression and PFS or OS (log-rank test)