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. 2016 Jan 28;7(1):e2074. doi: 10.1038/cddis.2015.402

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CDC27 promotes proliferation of CRC cells. (a) The efficient overexpression or suppression of CDC27 was identified by western blotting. (b) Representative images show the colony formation ability of HCT116 cells with CDC27 transiently suppressed. The number of the colonies was quantified. ***P<0.001 using Student's t-test. (c) Soft agar colony formation assays. Representative images are shown; the numbers of colonies containing more than 50 cells were scored. ***P<0.001 using Student's t-test. (d and e) CDC27 overexpression promoted cell growth in both colony formation assays and soft agar colony formation assays. Representative images are presented. *P<0.05 using Student's t-test. These experiments were repeated at least three times. Error bars, mean±S.D.