Figure 2.

Protein levels of different Bcl-2 family members and the deubiquitinase USP9x in glioblastoma cell lines. (a) Cell lysates of established glioblastoma cell lines A172, T98G, U373, and Ln229 as well as primary glioblastoma cells WKI and LKI and Jurkat T-lymphoma cells were separated by gel electrophoresis. Protein levels of the antiapoptotic Mcl-1, Bcl-2, Bcl-xL, and the pro-apoptotic Bax, Bak, Noxa, Puma, Bim, and Bad were analyzed by western blot. In addition, protein levels of the Mcl-1-stabilizing deubiquitinase USP9x were determined in all cell lines. β-actin was used as loading control. Mcl-1 and USP9x protein levels were higher in glioblastoma cells than in Jurkat T-lymphoma cells. (b) Densitometric analysis of USP9x and Mcl-1 was performed. Protein levels of Mcl-1 (upper panel) and USP9x (lower panel) were normalized to β-actin and then to these of Jurkat T cells. Results show mean values±S.D. (n=3). No significant regulation was observed