Figure 8.

Downregulation of USP9x by siRNA increased clonogenic death of irradiated A172 and Ln229 cells. In total, 3000 cells were seeded per well and transfected with 100 nM siRNA-targeting USP9x (usp9x) or non-targeting (nt) siRNA 24 h later. After 48 h of transfection, A172 and U373 cells were irradiated with 0–5 Gy. Colonies were allowed to form for 8–10 days. After counting the colonies, the surviving fraction (SF) was calculated. Surviving curves in response to IR are shown in (a), whereas representative wells are shown in (b). USP9x knockdown decreased the respective surviving fractions and, thus, increased clonogenic cell death in A172 and Ln229 cells, but showed no significant effect in U373 cells. Data show mean SF values±S.D. of one experiment that was performed in triplicates. One representative experiment of two is shown