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. 2016 Jan 28;7(1):e2073. doi: 10.1038/cddis.2015.412

Figure 3.

Figure 3

ADCC-mediated ROS accumulation is attenuated by exogenous antioxidants and XIAP overexpression. (a) Percent cell lysis of SUM149 cells incubated with cetuximab, PBMC alone or the combination in the presence or absence of concanamycin A (CMA), a perforin inhibitor. Bars represent mean±S.E.M. calculated percent lysis, n=2–3. (b) Percent cell lysis of SUM149 cells incubated with cetuximab alone or in ADCC conditions in the presence or absence of qVD (pan-caspase inhibitor), MnTBAP (antioxidant) or the combination. Bars represent mean±S.E.M. calculated percent lysis, n=2–3. *P<0.05 **P<0.005 (compared with vehicle). (c) Representative histograms of cells treated with: granzyme B (gB) alone (red line), streptolysin O (SLO) alone (blue line) or the combination of SLO+gB (green line) compared with untreated (black line). Number represents %positive for SLO+gB condition. Inset: Labeling of axes and diagram of sample colors, representative of n=2 experiments