Figure 2.

Pdcd4 deficiency enhanced autophagy-mediated breakdown of lipid in LD. Primary peritoneal macrophages from WT or pdcd4^−/− mice were stimulated by 50 μg/ml ox-LDL for 24 h. LD was stained with Bodipy to demonstrate neutral lipid (green) and anti-Lc3 conjugated with Alexa 555 to show autophagosome (red, a). The numbers of Lc3b⁺ autophagy puncta and Bodipy⁺ LDs per cell were accounted (b) and LD stained by Bodipy (green) was colocalized by lysosome, stained by LysoTracker Red (red, c). Real-time PCR was applied to show Abca1, Abcg1 and Lxr-α expression in macrophages (d). Data were representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001