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. 2015 Oct 20;309(12):E995–E1007. doi: 10.1152/ajpendo.00267.2015

Fig. 4.

Fig. 4.

A: effects of ROCK1 inhibitor H-1152 (1–3 μmol/l) on PDGF-BB-induced proliferation (cell number) of HASMCs. Data are means ± SE; n = 3 experiments in triplicates. B: microarray analysis of transcriptional changes in HASMCs treated with 2-ME (3 μmol/l) for 30 h. Expression levels are depicted as color scale from red (upregulation) to blue (downregulation). Gene Set Enrichment Analysis (GSEA) revealed upregulation of key RhoA/ROCK1 pathway-associated transcripts. C: significant inhibitory effects of 2-ME (3 μmol/l) on FCS (5%)-induced expression of GTP-bound RhoABC in HASMCs. D: inhibitory effects of 2-ME on PDGF-BB (20 ng/ml)-induced membrane-bound RhoA (m-RhoA) expression in HASMCs. Data are presented as means ± SE; n = 3. *P < 0.05 vs. vehicle-treated control; §significant inhibition vs. mitogen-treated cells.