Fig. 5.
A: time-dependent stimulatory effects of PDGF-BB (20 ng/ml) on expression of ROCK1 and inhibitory effects of 2-ME and H-1152 (ROCK1 inhibitor) on PDGF-BB-induced expression of ROCK1 in HASMCs. Data are presented as means ± SE; n = 3. *P < 0.05 vs. vehicle treated control. B: time-dependent stimulatory effects of PDGF-BB (20 ng/ml) on phosphorylation of MYPT and inhibitory effects of 2-ME and H-1152 on PDGF-BB-induced phosphorylation of myosin-targeting subunit of myosin light-chain phosphatase (MYPT) in HASMCs. C: inhibitory effects of 2-ME on angiotensin II-induced phosphorylated MYPT expression in HASMCs. Data are presented as means ± SE; n = 3.