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. 2015 Oct 20;309(12):E995–E1007. doi: 10.1152/ajpendo.00267.2015

Fig. 6.

Fig. 6.

A: time-dependent stimulatory effects of PDGF-BB (20 ng/ml) on phosphorylation of rMLC and inhibitory effects of 2-ME and H-1152 (ROCK1 inhibitor) on PDGF-BB-induced phosphorylation of regulatory subunit of myosin light chain (rMLC) in HASMCs. Data are presented as means ± SE; n = 3. *P < 0.05 vs. vehicle-treated control; §significant inhibition vs. mitogen-treated cells. B: representative fluorescent staining of HASMCs for inhibitory effects of 2-ME or H-1152 on PDGF-BB (20 ng/ml)-induced phosphorylation of rMLC (green fluorescence) and internal control nonphosphorylated rMLC (pink fluorescence). Three separate experiments were conducted, each in triplicate.