Table 1.
List of antibodies, chemicals, and buffers used in this study
| Antibodies, Chemicals, Buffers | Host | Company | Dilution |
|---|---|---|---|
| Primary antibodies | |||
| Anti-β-actin | Mouse | Sigma | 1:5,000 |
| Anti-Akt | Rabbit | Cell Signaling | 1:1,000 |
| Anti-phospho-Akt (Thr308) | Rabbit | Cell Signaling | 1:1,000 |
| Anti-cyclin B1 | Mouse | Upstate | 1:1,000 |
| Anti-cyclin D1 | Mouse | Upstate | 1:1,000 |
| Anti-ERK1/2 | Mouse | Upstate | 1:1,000 |
| Anti-phospho-ERK1/2 (Thr202/Tyr204) | Rabbit | Upstate | 1:1,000 |
| Anti-rMLC | Rabbit | Cell Signaling | 1:1,000 |
| Anti-phospho-rMLC (Ser19) | Mouse | Cell Signaling | 1:1,000 |
| Anti-phospho-rMLC (Ser19) | Rabbit | Cell Signaling | 1:1,000 |
| Anti-MYPT | Rabbit | Santa Cruz Biotechnology | 1:200 |
| Anti-phopsho-MYPT (Thr696) | Rabbit | Santa Cruz Biotechnology | 1:200 |
| Anti-RhoA | Mouse | Santa Cruz Biotechnology | 1:500 |
| Anti-ROCK | Rabbit | Santa Cruz Biotechnology | 1:500 |
| Anti-tissue factor | Rabbit | American Diagnostic | 1:1,000 |
| Secondary antibodies: target | |||
| IRDye 800 conj. anti-mouse IgG | Goat | LI-COR Biosciences | 1:12,500 |
| IRDye 800 conj. anti-rabbit IgG | Goat | LI-COR Biosciences | 1:12,500 |
| IRDye 680 conj. anti-rabbit IgG | Goat | LI-COR Biosciences | 1:12,500 |
| IRDye 680 conj. anti-mouse IgG | Goat | LI-COR Biosciences | 1:12,500 |
| IGF-peroxidase conj. anti-mouse IgG | Goat | Pierce | 1:100,000 |
| IGF-peroxidase conj. anti-rabbit IgG | Goat | Pierce | 1:100,000 |
| FITC conj. anti-mouse IgG | Goat | Sigma | 1:1,000 |
| TRITC conj. anti-rabbit IgG | Goat | Sigma | 1:1,000 |
| Chemicals and buffers | |||
| Thrombin | Sigma | ||
| TNF-α | R&D Systems | ||
| Angiotensin II | Sigma | ||
| Aprotinin | Sigma | ||
| APS (ammoniumpersulfate) | Sigma | ||
| BCA (bicinchoninic acid) protein assay kit | Pierce | ||
| BSA (bovine serum albumin) | Sigma | ||
| DAPI (4′-6-diamidino-2-phenylindole) | Sigma | ||
| GGPP (geranylgeranyl pyrophosphate) | Sigma | ||
| H-1152 | Enzo Life Sciences | ||
| Leupeptin (hemisulfate salt) | Sigma | ||
| Loading buffer (5x) | Fermentas, H | ||
| Lysis buffer | Cell Signaling Technology | ||
| 2-ME (2-methoxyestradiol) | Steraloids | ||
| MTT (thiazolyl blue tetrazolium bromide) | Sigma | ||
| PFA (paraformaldehyde) | Merck, Darmstadt | ||
| PBS (phosphate-buffered saline) | Gibco-BRL | ||
| PDGF-BB | Sigma | ||
| PMSF (phenylmethylsulfonyl fluoride) | Sigma | ||
| Ponceau S solution (2%) | Sigma | ||