Fig. 1.

Sepsis-induced alternations in regulated in development and DNA damage (REDD)1 and REDD2 mRNA and protein from wild-type (WT) and REDD1^−/− mice. REDD1 mRNA content (A) and protein expression (B) as well as REDD2 mRNA content (C) were determined by quantitative real-time PCR and Western blot analysis of gastrocnemius. REDD1 mRNA was nondetectable (ND) in REDD1^−/− mice. Gray bars represent WT mice (n = 10 sham and 15 septic), and black bars correspond to REDD1^−/− mice (n = 10 sham and 13 septic). Horizontal bars indicate statistical differences between groups (P < 0.05). Values are expressed as means ± SE.