Fig. 5.

Alterations in the phosphorylation of eukaryotic elongation factor (eEF)-2, eEF2 kinase (eEF2K), and AMP kinase (AMPK) in skeletal muscle in response to REDD1 deletion and sepsis. Bar graphs represent the quantification of Western blot images relative to the total amount of the respective protein eEF2 Thr⁵⁶ (A), eEF2K Ser³⁶⁶ (B), eEF2K Ser³⁹⁸ (C), and AMPK Thr¹⁷² (D) phosphorylation. Gray bars represent WT mice (n = 10 sham and 15 septic), and black bars correspond to REDD1^−/− mice (n = 10 sham and 13 septic). All values are expressed relative to sham-WT value, which was set to 100%. There was no significant genotype or sepsis effect on the total amount of any of the 4 proteins (data not shown). Horizontal bars indicate statistical differences between groups (P < 0.05). Values are expressed as means ± SE. Representative Western blots for the 4 treatment groups are shown.