Table 1.
Effect of sepsis in WT and REDD1−/− mice on body weight and composition, plasma glucose, lactate, and insulin concentrations
| Sham-WT | Sham-REDD1−/− | Septic-WT | Septic-REDD1−/− | |
|---|---|---|---|---|
| Body weight, g | ||||
| Presurgery (initial) | 22.9 ± 0.7 | 23.9 ± 0.6 | 23.1 ± 0.4 | 23.7 ± 0.4 |
| Postsurgery/sepsis | 20.9 ± 0.7 | 21.3 ± 0.5 | 21.5 ± 0.3 | 22.0 ± 0.4 |
| Body composition presurgery, % | ||||
| Fat mass | 7.6 ± 0.5 | 6.7 ± 0.5 | 7.3 ± 0.4 | 7.3 ± 0.4 |
| Lean mass | 73.6 ± 0.8 | 74.2 ± 0.6 | 73.4 ± 0.7 | 74.8 ± 0.3 |
| Muscle weight postsurgery/CLP, mg | ||||
| Gastrocnemius | 108 ± 3 | 107 ± 3 | 108 ± 3 | 106 ± 2 |
| Quadriceps | 136 ± 6 | 144 ± 5 | 138 ± 3 | 139 ± 5 |
| Plasma concentrations | ||||
| Glucose, mg/dl | 162 ± 4a | 168 ± 8a | 84 ± 5b | 106 ± 5c |
| Lactate, mg/dl | 53 ± 4a | 54 ± 3a | 87 ± 3b | 68 ± 2a,b |
| Insulin, ng/ml | 0.52 ± 0.07a | 0.80 ± 0.10a | 0.59 ± 0.13a | 1.46 ± 0.24b |
Values are expressed as means ± SE; n = 10, 10, 15, and 13 for Sham-WT, Sham-REDD1−/−, Septic-WT, and Septic-REDD1−/−, respectively. WT, wild type; CLP, cecal ligation and puncture. Prior to surgery and CLP, body weight and body composition (1H-NMR) were determined. Sepsis was then induced by CLP, and body weight was determined 24 h later at the time of euthanization. At this time point, muscle weight (right gastrocnemius and quadriceps) and blood were collected for the determination of plasma glucose, lactate, and insulin concentrations. Statistical differences between groups (P < 0.05) are indicated by different letters.