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. Author manuscript; available in PMC: 2016 Jul 8.
Published in final edited form as: Science. 2015 Nov 5;351(6269):aab2116. doi: 10.1126/science.aab2116

Figure 1. Use of novel phenotypic markers revealed heterogeneity in human hematopoietic hierarchy.

Figure 1

(A) A review of classic interpretations from previous work using CFC assays to measure the clonal outputs from a sorted, marker-pure population of cells. In scenario 1, each cell within a marker-pure population has the potential to give rise to three functional outputs (a, b, and c), but only gives rise to one of them in the assay. In this scenario, diverse functional outputs from a marker-pure population are interpreted to be derived from a pure population of a multilineage cells. In the alternate scenario 2, a marker pure population is comprised of three distinct unipotent cell types that give rise to their respective functional progeny. Solutions to each one of these scenarios are presented on the right. (B) The gating scheme of defining MPPs (CD34+CD38−Thy1−CD45RA−CD49f−), CMPs (CD34+CD38+CD10−FLT3+CD45RA−) and MEPs (CD34+CD38+CD10−FLT3CD45RA−) from FL is shown with black-dashed arrows. Each one of these compartments was further divided into F1 (CD71−BAH1−), F2 (CD71+BAH1−), and F3 (CD71+BAH1+) shown with blue-dashed arrows. Full gating scheme for FL, CB, and BM CD34+ cells is presented in fig. S1. (C) Summary of the new subsets used in this study.