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. 2015 Nov 23;36(12):1480–1486. doi: 10.1038/aps.2015.119

Figure 3.

Figure 3

miR-204 and miR-211 directly target recognition motifs in PPM1K 3′UTR. (A) Schematic diagram showing the seed sequence of miR-204 and miR-211 and putative conserved recognition motif in human PPM1K 3′UTR. (B) Luciferase assay results from HeLa cells co-transfected with miRNA mimics and luciferase reporter vectors. Luc-human 3′UTR-D is the mutated reporter vector where the conserved recognition motif was deleted in human_PPM1K 3′UTR. (C) Schematic diagram showing the seed sequences of miR-204 and miR-211, with putative matched conserved and poorly conserved sequences in mouse Ppm1k 3′UTR. (D) Luciferase assay results from HeLa cells. Luc-mouse 3′UTR-D1 is the mouse Ppm1k 3′UTR reporter vector with conserved site deleted. Luc-mouse 3′UTR-D2 is the mouse Ppm1k 3′UTR reporter vector with less-conserved site deleted. Luc-mouse 3′UTR-DD is the mouse Ppm1k 3′UTR reporter vector with both sites deleted. Data are mean±SEM for three individual samples (bP<0.05).