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. 2015 Jun 11;6(6):e89. doi: 10.1038/ctg.2015.20

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Copyright © 2015 American College of Gastroenterology

Clinical and Translational Gastroenterology is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Analysis of the operational taxonomic unit (OTU) number. (a) The rarefaction curve plots the number of OTU as a function of the number of reads. Squares, circles and triangles represent the curves for salivary, gastric fluid (GF), and fecal micobiota, respectively. (b and c) The OTU number representing the bacterial species richness of the microbiota was estimated using the (b) observed OTU and the (c) Chao 1 index.