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. 2015 Jul 2;6(7):e95. doi: 10.1038/ctg.2015.23

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Copyright © 2015 American College of Gastroenterology

Clinical and Translational Gastroenterology is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Impact of LIGHT (homologous to lymphotoxin, exhibits inducible expression, and competes with HSV glycoprotein D for herpes virus entry mediator (HVEM), a receptor expressed by T lymphocytes) on the expression of interleukin-8 (IL-8) in Huh7 (human hepatoma cell line) hepatocytes. Effect of recombinant human LIGHT (ng/ml) on the expression of IL-8 in Huh7 hepatocytes after (a and b) 2 h and (c and d) 8 h stimulation. Left panels show mRNA levels of IL-8 from cell lysates, as assessed by real-time reverse transcription-PCR (RT-PCR), in relation to the control gene β-actin, and right panels show protein levels of IL-8 measured in the supernatant from cells by enzyme immunoassay. Data are given as mean±s.e.m., n=6. *P=0.06, *P<0.05, **P<0.01, and ****P<0.0001 vs. US (unstimulated).