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Peptide specific for EGFR. (a) Chemical structure of QRHKPRE peptide (black) with GGGSK linker (blue) and Cy5.5 fluorophore (red). (b) Scrambled peptide PEHKRRQ (control). (c) QRH*-Cy5.5 was found on the structural model to bind domain 2 of EGFR (1IVO). (d) Fluorescence spectra of Cy5.5-labeled peptides with λex=671 nm shows peak emission near 710 nm. AU, arbitrary unit; EGFR, epidermal growth factor receptor.
