Table. Ig detection by immunofluorescent assay and molecular results by PCR and sequence identity of the amplicons of gltA and 17-kDa genes of Rickettsia spp. from 2 patients with murine typhus, Yucatan, Mexico.
Patient age, y/sex | Days after illness onset | Immunofluorescence assay |
Rickettsia species* | |||||||
---|---|---|---|---|---|---|---|---|---|---|
R. typhi
|
R. rickettsii
|
PCR/sequence test |
||||||||
IgM | IgG | IgM | IgG | 17 kDa | gltA | |||||
45/F | 8 | 256 | 128 | Neg | 128 | Pos | Pos | R. typhi | ||
12 | 128 | 128 | Neg | 64 | Pos | Pos | R. typhi | |||
|
16 |
64 |
128 |
|
Neg |
64 |
|
Neg |
Neg |
ND |
12/M | 8 | 128 | 128 | Neg | 64 | Pos | Pos | R. typhi |
*Identified species showed 99% identity with R. typhi Wilmington strain 17-kDa antigen gene (GenBank accession no. AE017197.1) and 100% identity with R. typhi Wilmington strain gltA gene (GenBank accession no. AE017197.1). ND, species not determined.