Table 3. The identification of differentially responsive proteins in exogenous ABA pre-treated tea leaves subjected to drought.
| Spot no. | Protein name | Accession no.a | Species | Theoretical |
Experimental |
Scoreb | %cov (matching peptides)c | ||
|---|---|---|---|---|---|---|---|---|---|
| Mr (kDa) | pI | Mr (kDa) | pI | ||||||
| 1 | Apolipoprotein d, putative | XP_002523505 | Ricinus communis | 21.63 | 6.33 | 16.78 | 6.63 | 55 | 11% (2) |
| 2 | Chloroplast phosphoribulokinase | BAL03266 | Closterium peracerosum-strigosum-littorale complex | 45.77 | 6.28 | 55.07 | 5.67 | 129 | 12% (3) |
| 3 | Cytochrome B6-F complex iron sulfur subunit 2 | ABQ41994 | Sonneratia caseolaris | 16.83 | 5.75 | 18.84 | 5.61 | 220 | 28% (3) |
| 4 | Fructose-biphosphate aldolase | ACG68894 | Sesuvium portulacastrum | 38.69 | 6.49 | 37.27 | 6.50 | 45 | 6% (1) |
| 5 | High molecular weight heat shock protein | AAF34134 | Malus × domestica | 71.57 | 5.17 | 60.23 | 5.42 | 454 | 11% (3) |
| 6 | Predicted: low-quality protein: aldehyde dehydrogenase family 2 member B4, mitochondrial-like | XP_003528154 | Glycine max | 59.02 | 6.30 | 68.63 | 6.28 | 65 | 4% (2) |
| 7 | Predicted: protein IN2-1 homolog B-like | XP_003554349 | Glycine max | 27.05 | 5.49 | 41.20 | 5.54 | 73 | 5% (1) |
| 8 | S-adenosylmethionine synthase 3 | P50303 | Actinidia chinensis | 39.83 | 6.20 | 64.00 | 6.16 | 342 | 23% (4) |
| 9 | Short chain dehydrogenase/reductase | ACN87274 | Chelidonium majus | 32.98 | 4.86 | 41.04 | 6.35 | 66 | 5% (2) |
| 10 | Rubisco activase | ABU49419 | Camellia sinensis | 15.72 | 5.85 | 61.25 | 5.75 | 96 | 20% (2) |
| 11 | Ribulose bisphosphate carboxylase activase B | AAF71272 | Triticum aestivum | 48.01 | 6.92 | 73.51 | 5.48 | 466 | 18% (6) |
| 12 | Ribulose bisphosphate carboxylase large chain | P28426 | Ilex crenata | 51.97 | 6.10 | 20.80 | 6.56 | 343 | 12% (6) |
| 13 | Ribulose bisphosphate carboxylase large chain | P28426 | Ilex crenata | 51.97 | 6.10 | 35.11 | 6.65 | 346 | 10% (5) |
| 14 | Ribulose bisphosphate carboxylase large chain | P25830 | Cerastium glomeratum | 53.20 | 6.22 | 33.32 | 6.72 | 352 | 14% (4) |
| 15 | Ribulose 1,5-bisphosphate carboxylase | CAQ58398 | Prismatomeris sp. 1 Davis 4057 | 52.12 | 6.34 | 15.08 | 5.52 | 225 | 10% (4) |
| 16 | Ribulose 1,5-bisphosphate carboxylase | CAQ58398 | Prismatomeris sp. 1 Davis 4057 | 52.12 | 6.34 | 19.87 | 6.70 | 260 | 12% (5) |
| 17 | Ribulose 1,5-biphosphate carboxylase large subunit | AAM33278 | Stachys affinis | 50.19 | 6.78 | 41.26 | 6.45 | 399 | 18% (5) |
| 18 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | CAB02249 | Maesa myrsinoides | 51.65 | 6.34 | 68.80 | 6.70 | 422 | 25% (8) |
| 19 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | AAA21212 | Aruncus dioicus | 52.13 | 6.04 | 16.02 | 5.17 | 103 | 4% (1) |
| 20 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | CAB02254 | Stewartia pseudocamellia | 51.72 | 6.46 | 18.49 | 6.28 | 426 | 11% (4) |
| 21 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | AAK61184 | Nyssa yunnanensis | 49.87 | 5.95 | 12.60 | 5.25 | 48 | 5% (2) |
| 22 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | CAB00004 | Margaritaria tetracocca | 52.03 | 6.04 | 15.01 | 5.53 | 262 | 10% (4) |
| 23 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | AAK61184 | Nyssa yunnanensis | 49.87 | 5.95 | 12.62 | 5.21 | 48 | 5% (2) |
| 24 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | AAD29404 | Dactylopetalum ellipticifolium | 49.56 | 6.46 | 11.03 | 5.28 | 114 | 6% (2) |
| 25 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | AAV65404 | Apodiscus chevalieri | 51.64 | 6.04 | 38.92 | 6.53 | 465 | 18% (5) |
| 26 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | AAB67920 | Securigera varia | 52.12 | 6.04 | 13.68 | 5.21 | 232 | 9% (3) |
| 27 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | AFI23110 | Psychotria graciliflora | 20.58 | 7.63 | 17.94 | 5.27 | 143 | 31% (1) |
| 28 | Predicted: ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic | XP_002282979 | Vitis vinifera | 48.21 | 5.50 | 53.47 | 5.68 | 398 | 16% (4) |
| 29 | Chloroplast ribulose 1,5-bisphosphate carboxylase/oxygenase activase | ABW80752 | Flaveria bidentis | 48.81 | 6.10 | 41.32 | 5.18 | 285 | 17% (6) |
| 30 | Predicted: uncharacterized protein LOC100251175 isoform 1 | XP_002271111 | Vitis vinifera | 19.01 | 4.53 | 21.71 | 5.15 | 179 | 20% (2) |
| 31 | Predicted protein | XP_002306773 | Populus trichocarpa | 41.20 | 6.67 | 53.28 | 6.71 | 203 | 9% (3) |
| 32 | Unknown | ACU23262 | Glycine max | 33.46 | 6.84 | 11.65 | 5.75 | 58 | 4% (1) |
| 33 | Unnamed protein product | CBI23038 | Vitis vinifera | 25.28 | 6.82 | 22.75 | 5.59 | 117 | 21% (3) |
a
Accession number in NCBI database
b
Score is a measure of the statistical significance of a match
c
Percentage of predicted protein sequence covered by matched peptides