Table 2. The effect of DMSO on white blood cell subtypes stimulated with E. coli in the human whole blood assay.
| Control | E. coli | 0.25% | 0.5% | 1% | 2% | 3% | 5% | 10% | |
|---|---|---|---|---|---|---|---|---|---|
| Granulocytes | 56.2 ± 1.1 | 55.2 ± 2.3 | 52.8 ± 1.7 | 47.8 ± 1.4* | 48.7 ± 2.2* | 46.9 ± 7.8 | 52.5 ± 3.0 | 58.2 ± 0.7 | 27.1 ± 1.2* |
| Lymphocytes | 32.1 ± 0.4 | 34.6 ± 1.9 | 36.6 ± 1.5 | 41.0 ± 1.2* | 39.1 ± 1.6* | 43.7 ± 8.1 | 36.6 ± 2.3 | 27.8 ± 0.8* | 15.6 ± 1.0* |
| Monocytes | 7.9 ± 0.3 | 6.8 ± 0.3 | 7.8 ± 0.5* | 7.8 ± 0.7* | 6.1 ± 0.2* | 3.7 ± 0.5* | 1.6 ± 0.2* | 2.0 ± 0.3* | 1.8 ± 1.3* |
* denotes significant (P <0.05) difference between DMSO-treated and untreated cells. Data are expressed as % of total cells mean ± SD) of triplicates from a representative experiment from two independent studies. The cells were subtyped via flow cytometry, based on their forward and side scatter properties.