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. 2016 Mar 31;11(3):e0152626. doi: 10.1371/journal.pone.0152626

Fig 3. Co-expression of PfAdoMetDC with various chaperone sets in E. coli BL21 (DE3) Star cells.

Fig 3

Whole cell lysates from the co-expression of PfAdoMetDC with chaperone sets (A) DnaK + DnaJ, (B) KPf + DnaJ, (C) PfHsp70 + DnaJ, (D) DnaK + GroEL + DnaJ, (E) KPf + GroEL + DnaJ, and (F) PfHsp70 + GroEL + DnaJ were analysed on SDS-PAGE (upper panel) and confirmed by Western blot analysis (lower panels). Lanes U–uninduced whole cell lysate; I–whole cell lysate 1 hour post IPTG (1 mM) induction; A1 –whole cell lysate 1 hour post AHT (2 ng/ml) induction; A2 –whole cell lysate 2 hours post AHT plus IPTG induction. Lower panels: DnaK was detected using α-DnaK antibody, PfHsp70 was detected using α-PfHsp70 antibody and PfAdoMetDC (60 kDa) was detected using α-Strep antibody. Numbers to the left represent protein markers (Fermentas) in kDa.