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. 2016 Mar 31;11(3):e0152626. doi: 10.1371/journal.pone.0152626

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© 2016 Makhoba et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) SDS-PAGE (top panel) and Western (lower panels) representing the purification of PfAdoMetDC from E. coli ΔdnaK cells (lane ΔdnaK) and BL21 Star (DE3) cells (lane DnaK), respectively. PfAdoMetDC (60 kDa) and DnaK (70 kDa) were detected using α-Strep and α-DnaK antibodies, respectively. Numbers to the left represent protein markers (Fermentas) in kDa.