Table 1.
IHC staining patterns in normal skin
| Antigen | Antibodies | Internal control | Figure |
|---|---|---|---|
| Pan–cytokeratin | AE1/3 (AE1–acidic CK; AE3–basic CK), Pan–K | suprabasal keratinocytes | 1a, b, f |
| HMWCK | 34βE12 (CK1, 5, 10, 14, 15), CK5/6 | suprabasal keratinocytes and adnexal epithelia | 1c, d |
| LMWCK | CAM5.2, CK7 | secretory portion of eccrine and apocrine glands | 1e |
| Muscle markers | SMA, desmin | pilar smooth muscle | 2a–d |
| Eccrine and apocrine glands | CEA, EMA | sweat glands | 2e, f |
| Endothelial cell markers | CD31, CD34, vWF, ERG–1 | endothelial cells in vessels | 3a–d |
| Melanocytic markers | S100, MART–1, MITF, HMB–45, tyrosinase | epidermal basal layer melanocytes | 4 |
| Langerin | CD1a, CD4 | epidermal Langerhans cells | |
| Axon | NPF, S100 | nerve bundle | |
LMWCK = Low–molecular–weight cytokeratin; NPF = neuropeptide F; vWF = von Willebrand factor; Pan–K = pan–keratin.