Table 2. Minimal inhibitory concentrations (mg/L) of Sporothrix spp. strains in presence of the melanin inhibitors tricyclazole, glyphosate, and sulcotrione.
| Strain | Amphotericin B | Itraconazole | Ketoconazole | Terbinafine | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PDA | Tria | Glyb | Sulc | PDA | Tri | Gly | Sul | PDA | Tri | Gly | Sul | PDA | Tri | Gly | Sul | |
| S. brasiliensis | ||||||||||||||||
| CBS 120339 | 2.0 | 2.0 | 2.0 | 2.0 | 1.0 | 0.5 | 1.0 | 1.0 | 0.5 | 0.12* | 0.5 | 0.5 | 0.06 | 0.015* | 0.06 | 0.06 |
| IPEC 26449 | 1.0 | 2.0 | 1.0 | 1.0 | 1.0 | 0.5 | 1.0 | 1.0 | 0.5 | 0.25 | 0.5 | 0.5 | 0.03 | 0.008* | 0.015 | 0.015 |
| S. schenckii | ||||||||||||||||
| IPEC 24372 | 8.0 | 4.0 | 4.0 | 4.0 | 1.0 | 1.0 | 1.0 | 1.0 | 0.5 | 0.25 | 0.5 | 0.25 | 0.03 | 0.015 | 0.03 | 0.03 |
| IPEC 23250 | 8.0 | 4.0 | 2.0 | 2.0 | 1.0 | 0.5 | 0.5 | 0.5 | 0.5 | 0.25 | 1.0 | 0.25 | 0.03 | 0.015 | 0.03 | 0.03 |
| ATCC 16345 | 4.0 | 4.0 | 2.0 | 2.0 | 8.0 | 4.0 | 2.0* | 2.0* | 1.0 | 1.0 | 2.0 | 2.0 | 0.12 | 0.015* | 0.06 | 0.06 |
| ATCC 32268 | 8.0 | 4.0 | 4.0 | 4.0 | 1.0 | 1.0 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.06 | 0.015* | 0.06 | 0.03 |
a Tri, RPMI-1640 medium with the antifungal drugs supplemented with tricyclazole
b Gly, RPMI-1640 medium with the antifungal drugs supplemented with glyphosate
c Sul, RPMI-1640 medium with the antifungal drugs supplemented with sulcotrione
*indicates a greater than one fold dilution difference in MIC between the different inhibitor conditions.