Fig 1. Schematic representation of the DSS study.

Lambs (n = 14) were inoculated with Clone 13 via SC and ID route. A group of the same size functioned as sentinels. On days 2, 4, 6, 8, 10, 12 and 14, two lambs from each group were euthanized. The following samples were collected for detection of Clone 13: saliva, nasal mucus, lacrimal fluid, urine and faeces. During necropsy, gonades, kidney, adrenal gland, spleen, Ln. ileocaecalis, small and large intestines, bladder, pancreas, liver, lung, thymus, heart muscle, injection sites, M. tripceps brachii, Ln prescapularis left and right, salivary gland and brain samples were collected.