Table 1. Inoculation routes and Clone 13 titers used in this study.

Study	Inoculation route1 and volume	Titer of inoculum [log10TCID50/ml]
DSS	SC 1.0 ml	7.9
	ID 0.2 ml
RTV	SC 1.0 ml	7.4
	ID 0.1 ml
Safety in lambs first vaccination	SC 2.0 ml	7.6
	ID 0.2 ml
	IN 2x 0.5 ml	7.1
	IO 2x 0.5 ml
Safety in lambs second vaccination	SC 1.0 ml	7.1
	ID 0.1 ml
	IN 2x 0.5 ml	7.1
	IO 2x 0.5 ml
Safety in pregnant ewes (~50 days after mating)	SC 1.0 ml	6.8
	ID 0.1 ml
Safety in pregnant ewes (~120 days after mating)	SC 1.0 ml	7.0
	ID 0.1 ml

¹SC; subcutaneous, ID; intradermal, IN; intranasal, IO; intraocular