Table 2. Detection of Clone 13 by RT-qPCR in samples taken from lambs during the dissemination, shedding and spreading (DSS) study1.
| Animal no. | Plasma2 dpi | Necropsy on dpi | Injection site | Ln prescapularis | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Spleen | Liver | left (SC) | right (ID) | left | Right | |||||
| 3164 | - | 2 | - | - | 6.2 | - | - | 6.8 | ||
| 3184 | 4.8 | 2 | - | 4.4 | 8.0 | 4.0 | 6.8 | 4.7 | ||
| 3178 | 4.3 | 4 | - | - | - | 6.5 | 6.6 | 7.0 | ||
| 3179 | 4.6 | 4 | - | - | 6.6 | - | 7.5 | 7.5 | ||
| 3163 | 4.4 | 6 | - | - | - | 6.0 | 5.6 | 7.1 | ||
| 3168 | 4.5 | 6 | - | - | - | - | 7.0 | 6.4 | ||
| 3161 | 4.7 | 8 | 4.7 | 3.5 | 4.5 | 3.6 | 6.2 | 6.5 | ||
| 3182 | 4.6 | 8 | - | - | - | - | 7.8 | 7.7 | ||
| 3169 | 4.5 | 10 | 4.4 | - | 3.9 | - | 3.9 | 5.4 | ||
| 3165 | 4.4 | 10 | - | - | 5.4 | 4.2 | - | - | ||
| 3172 | 3.8 | 12 | - | - | - | - | - | 5.7 | ||
| 3176 | 4.4 | 12 | - | - | 4.7 | 5.3 | 5.3 | 5.2 | ||
| 3174 | 4.8 | 14 | - | - | - | - | 4.5 | - | ||
| 3189 | 4.5 | 14 | - | - | - | - | - | - | ||
1RVFV specific RNA was measured by RT-qPCR in plasma samples taken two days after inoculation and from spleen, liver, both prescapular lymph nodes and the subcutaneous (SC) and the intradermal injection (ID) sites collected at necropsy at different days post inoculation (dpi). Results are depicted as log10 RNA copies/ml. A negative result in the RT-qPCR is indicated by the “-”sign. Samples from which virus was isolated are marked in bold. All samples collected from sentinel animals were RT-qPCR-negative and were therefore not included.