Fig 5. Replication kinetics of poliovirus infection of suspension PER.C6cells with a cell density of 10⁷ cells/ml at an MOI of 1 at 30°C and 37°C harvested between 0–48 hours post infection.

Viruses used were Brunenders, Brunenders—with 14 CAVA mutations, MEF-1, MEF-1 –with 14 CAVA mutations, Sabin 3, Sabin 3 –with 14 CAVA mutations and the CAVA backbone virus (see overview of all viruses in Fig 2). The Sabin 3/Sabin 3–14 infection was performed independently from the Brunenders/Brunenders-14 and MEF-1/MEF-1-14 infections. However, the control taken along (CAVA-backbone) was similar for both experiments.