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Rat anti-Leptospira antibody responses were measured by the micro-agglutination test. The box plot shows the log10 titers of the sera samples of eight rats from intraperitoneal (A), mucosal (B) and subcutaneous (C) groups (tested in triplicate at a starting dilution of 1:50). Leptospiral antibody levels in each group were only significantly different on day 7 (p-value < 0.05).
