Figure 3. Behavioral tests of fear.

(A) Representative recordings of “Startle Stimulus Only” and “Tone + Startle Stimulus” trials in a α2F/F control mouse treated with vehicle. The increased startle amplitude in “Tone + Startle” trials represents fear-potentiation of the startle response. (B-E) Mean ( ± S.E.M.) startle amplitude in “No Tone” and “Tone” startle trials in vehicle and diazepam-treated (B) α2F/F, (C) αCA1KO, (D) α2CA3KO, (E) α2DGKO mice. (F) Mean ( ± S.E.M.) percent FPS in trials preceded by the tone. Asterisks represent significant difference from the corresponding vehicle group. (G) Mean ( ± S.E.M.) number of licks recorded in the pretest session of the VCT where drinking is not punished (This session does not involve drug administration). (H) Mean ( ± S.E.M.) number of licks recorded in the test session where every 20th lick is punished in vehicle- or diazepam-treated mice. Asterisks represent significant difference from the corresponding vehicle group. *p<0.05, **p<0.01, ***p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.14120.014

Figure 3—figure supplement 1. Additional measures in tests of fear-related behavior.

(A-D) Mean (± S.E.M.) startle amplitude in response to different intensities of white noise bursts measured in arbitrary units based on the displacement of load cells during the first three days (Habituation sessions) of the FPS experiment in (A) α2F/F, (B) α2CA1KO, (C) α2CA3KO, (D) α2DGKO mice. (E) Mean ( ± S.E.M.) shock sensitivity measured by the average motion registered during 0.4 mA shocks. (F) Mean ( ± S.E.M.) number of unpunished licks on test day in vehicle- and diazepam-treated α2F/F control mice.

DOI: http://dx.doi.org/10.7554/eLife.14120.016