Table 1.
Results of omnibus statistical tests of experiments for the general characterization of α2CA1KO, α2CA3KO and α2DGKO mice.
| 1. Immunohistochemistry | ||||||
| One-Way ANOVA; Factor: Genotype | ||||||
| α2 Subunit | α1 Subunit | α5 Subunit | ||||
| CA1 | F(3,10)=9.44 | P=0.003 | F(3,9)=0.08 | p=0.97 | F(3,9)=0.10 | p=0.96 |
| CA3 | F(3,10)=5.05 | P=0.02 | F(3,9)=0.01 | p=0.99 | F(3,9)=0.13 | p=0.94 |
| DG | F(3,10)=9.08 | P=0.003 | F(3,9)=0.00 | p=1.00 | F(3,9)=0.06 | p=0.98 |
| Cortex | F(3,10)=1.90 | p=0.19 | F(3,9)=0.01 | p=0.83 | F(3,9)=0.01 | p=0.99 |
| Amygdala | F(3,10)=1.28 | p=0.34 | F(3,9)=0.21 | p=0.89 | F(3,9)=0.36 | p=0.78 |
| 2. Quantitative PCR | ||||||
| One-Way ANOVA; Factor: Genotype | ||||||
| α2 Subunit | α3 Subunit | α4 Subunit | ||||
| CA1 | F(3,16)=10.66 | p<0.001 | F(3,16)=0.10 | p=0.96 | F(3,16)=2.48 | p=0.1 |
| CA3 | F(3,16)=10.53 | p<0.001 | F(3,16)=0.67 | p=0.58 | F(3,16)=4.74 | p=0.02 |
| DG | F(3,16)=7.32 | p=0.003 | F(3,12)=1.96 | p=0.17 | F(3,12)=2.90 | p=0.08 |
| Cortex | F(3,16)=15.69 | p<0.001 | ||||
| Amygdala | F(3,16)=3.01 | p=0.06 | ||||
| 3. Slice Electrophysiology | ||||||
| Two-Way Mixed Factorial ANOVA; Factors: Genotype (between-subjects), Drug (within-subjects) | ||||||
| CA1 | Amplitude | Frequency | Decay Time | |||
| Genotype | F(1,38)=1.83 | p=0.18 | F(1,38)=2.80 | p=0.10 | F(1,38)=1.94 | p=0.17 |
| Drug | F(1,38)=1.49 | p=0.23 | F(1,38)=9.38 | p=0.004 | F(1,38)=107.96 | p<0.001 |
| Genotype x Drug Interaction | F(1,38)=2.91 | p=0.09 | F(1,38)=3.11 | p=0.08 | F(1,38)=0.82 | p=0.37 |
| CA3 | Amplitude | Frequency | Decay Time | |||
| Genotype | F(1,21)=2.36 | p=0.14 | F(1,21)=1.85 | p=0.19 | F(1,21)=0.95 | p=0.34 |
| Drug | F(1,21)=0.66 | p=0.42 | F(1,21)=0.09 | p=0.77 | F(1,21)=30.54 | p<0.001 |
| Genotype x Drug Interaction | F(1,21)=0.21 | p=0.65 | F(1,21)=1.90 | p=0.18 | F(1,21)=0.25 | p=0.62 |
| DG | Amplitude | Frequency | Decay Time | |||
| Genotype | F(1,21)=1.50 | p=0.23 | F(1,21)=1.58 | p=0.22 | F(1,21)=0.01 | p=0.91 |
| Drug | F(1,21)=2.58 | p=0.12 | F(1,21)=1.58 | p=0.22 | F(1,21)=47.42 | p<0.001 |
| Genotype x Drug Interaction | F(1,21)=0.54 | p=0.47 | F(1,21)=1.33 | p=0.26 | F(1,21)=0.30 | p=0.59 |
| 4. Tests of Hippocampal Function | ||||||
| Delay – Trace Fear Conditioning | ||||||
| Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Condition (between-subjects) | ||||||
| % Freezing | ||||||
| Genotype | F(3,49)=0.38 | p=0.77 | ||||
| Condition | F(1,49)=41.57 | p<0.001 | ||||
| Genotype x Cond. Interaction | F(3,49)=0.71 | p=0.55 | ||||
| Contextual Fear Conditioning | ||||||
| One-Way ANOVA; Factor: Genotype (between-subjects) | ||||||
| % Freezing | ||||||
| Genotype | F(3,35)=5.47 | p=0.003 | ||||
| Morris Water Maze | ||||||
| Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Day (within-subjects) | ||||||
| Time to platform | ||||||
| Genotype | F(3,80)=5.17 | p=0.01 | ||||
| Day | F(5,80)=244.12 | p<0.001 | ||||
| Genotype x Day Interaction | F(5,80)=1.36 | p=0.19 | ||||