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. 2016 Mar 29;110(6):1312–1321. doi: 10.1016/j.bpj.2016.01.026

Figure 3.

Figure 3

Cellular localization of BvPIP1;1-ECFP and BvPIP2;2-EYFP when coexpressed with heterodimer in Xenopus laevis oocytes. (A) Confocal images of an oocyte coexpressing the heterodimer with BvPIP1;1-ECFP in mass ratios of 1:2 (AI, green) and 1:1 (AII, green), or expressing BvPIP1;1-ECFP alone (AIII, green); oocytes were previously injected with TMR-dextran (red). (B) Confocal images of an oocyte coexpressing heterodimer with BvPIP2;2-EYFP in mass ratios of 1:2 (BI, green) and 1:1 (BII, green), or expressing BvPIP2;2-EYFP alone (BIII, green); oocytes were previously injected with TMR-dextran (red). The oocyte surface is near the right of each image frame, and the interior of the oocyte is to the left. (Insets) Enlargement of the indicated square section. Scale bar, 20 μm; inset scale bar, 5 μm. (C and D) Normalized mean intensity profiles of areas selected from each confocal image (dashed white rectangles). Graphs (CI)–(CIII) correspond to the mean intensity profiles of (AI)–(AIII), and graphs (DI)–(DIII) correspond to the mean intensity profiles of (BI)–(BIII), respectively. The black line corresponds to the ECFP or EYFP signal, and the red line to the TMR-dextran signal. IN/OUT indicates the interior/exterior of the cell, respectively. To see this figure in color, go online.