Figure 1.

(A) Schematic of DiaSLM. A dithered Gaussian lattice propagating in the Y-direction illuminates a cell oriented at 45°, and fluorescence is imaged in an orthogonal direction. The illumination beam is scanned diagonally in the S-direction along the coverslip, and an image is acquired at each Z plane. The beam length is configured to encompass the tallest region of the cell (e.g., the nucleus, ∼11 μm at FWHM). (B) Image of U2OS cells expressing CyOFP-Tractin, acquired at an intermediate Z plane. (C) A single plane in YZ, showing the 45° sample geometry and high degree of optical sectioning. (D) XY maximum intensity projection of the same U2OS cells expressing CyOFP-Tractin. Scale bar, 10 μm.