Figure 3.

Lack of leukocyte p47 is associated with progressive obstructive lung lesions. A: Representative micrographs of hematoxylin and eosin–stained lungs of Akita/Ncf1 male mice at ages 6, 8, 10, and 12 weeks show the progression of cellular lung infiltration over time. Alveolar spaces (asterisks) become progressively closed starting at age 8 weeks when cellular infiltrates are observed around arterioles. At 10 weeks, lungs present with a mixed infiltrate containing numerous alveolar macrophages (black arrow) and neutrophils (white arrow). Lung section from a 12-week-old Akita/Ncf1 mouse shows alveolar space obliterated by neutrophils (white arrow). B: Lung weights of male Akita/Ncf1 mice increase progressively from age 10 weeks. C: Immunohistochemical staining of CD162-positive cells (arrow) in the perivascular infiltrate (asterisk, blood vessel). D: Representative hematoxylin and eosin–stained section of female Akita/Ncf1 lungs at age 12 weeks showing perivascular (arrows) mononuclear cell infiltration. Perivascular infiltration at ×40 magnification. E: Representative histological lung sections from a 9-month-old Akita/Ncf1 female stained with hematoxylin and eosin illustrating accumulation of extracellular eosinophilic crystalline material (white arrow) in the lung parenchyma and inflammatory cell infiltration (black arrow). Inset shows eosinophilic crystalline material (black arrowhead, ×40 magnification). F: Periodic acid-Schiff staining of male Akita/Ncf1 lungs revealed fungal infection at 12 weeks (arrows) but not at ages 6, 8, and 10 weeks. G: PCR for 16S ribosomal RNA in lungs of male Akita/Ncf1 mice revealed no bacterial colonization at ages 6 to 12 weeks. n = 2 mice per group (B). Scale bar: 0.25 mm (D); 20 μm (A, C, E, and F).