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. 2016 Mar 29;110(6):1391–1399. doi: 10.1016/j.bpj.2015.11.3529

Figure 2.

Figure 2

Structural influences of pH on M2FL. Spectral slices obtained at 262 K from 13C-13C DARR spectra of 13C amino acid-labeled mixed M2 samples at pH 7.5 and 5.0. (A) Spectral slices through Val-Cα frequency (66.8 ppm) using a mixing time of 500 ms and a 10.8 kHz spinning rate. (Dashed line) Leu-Cα frequency (∼58 ppm). (B) Spectral slices through Ala-Cα frequency (55.8 ppm) using a mixing time of 300 ms and a 12.2 kHz spinning rate. (Dashed line) Ile-Cγ1 frequency (∼27 ppm). (C) Spectral slices through Leu-Cα frequency (58.0 ppm) using a mixing time of 500 ms and a 12.2 kHz spinning rate. (Dashed line) Ile Cα frequency (65 ppm). (D and E) Spectral slices through Leu-Cγ frequency (26.6 ppm) using a mixing time of 500 ms and a 9.0 kHz spinning rate. (Dashed lines) Phe-Cγ (∼138 ppm) and Phe-Cδε (∼130 ppm) frequencies.