Figure 3.

Comparison of CpG methylation (mCpG) levels in tumor and normal endometrial samples, as determined by amplicon sequencing. A: Box plots of percentage of mCpG at each CpG position within the amplicon in normal (empty black) and tumor (shaded gray) samples. Samples contained a minimum of 1000 aligned reads. B: Scatterplot of tumor (T) methylation levels measured with Illumina methylation arrays at probe cg21790626 (x axis) versus amplicon sequencing at the corresponding genomic position, chr19:58220494 (y axis), in the same samples. C: Scatterplot of the mean percentage of methylation across all amplicon CpG positions for each normal (N) sample, plotted against duplicate values.