Figure 4.

Distribution of individual CpG methylation (mCpG) levels in lung, stomach, colon, and breast tumor and normal tissue samples. A: Box plots of the mean percentage of methylation, determined from bisulfite sequencing, at each CpG position within the amplicon in normal (empty black) and tumor (shaded gray) samples. Samples contained ≥1000 aligned reads. B: Scatterplots of the mean percentage of methylation across all amplicon CpG positions for tumor (T) and normal (N) samples are plotted against duplicate values, when both duplicates have at least 1000 aligned reads.