Figure 5.

Methylation patterns of aligned reads in tumor versus normal endometrial, colon, stomach, lung, and breast tissue samples. A: Frequency of the 45 most repeated patterns. Unmethylated cytosines converted to thymines appear as (.), whereas methylated cytosines that were protected from conversion appear as (c). Each symbol represents the status of one of the 20 CpG cytosines in the amplicon. B: Hierarchical clustering of the samples based on these 45 patterns. Heat map coloring reflects the relative abundance of a given pattern across samples—going from white to black in each row or pattern would correspond to moving from the bottom upward in the merged tumor-and-normal box plot for that same pattern, similar to A.