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. 2016 Mar;18(2):260–266. doi: 10.1016/j.jmoldx.2015.11.003

Figure 1.

Figure 1

PCR and multiplex allele-specific primer extension (ASPE) primer locations for APOL1 genotyping and Sanger sequencing. The NG_023228 genomic sequence of the APOL1 gene (http://www.ncbi.nlm.nih.gov/nuccore; accession number NG_023228.1) was used to design all PCR and ASPE primers for APOL1 genotyping and Sanger sequencing. The APOL1 genotyping and Sanger sequencing primer pairs used for amplification were PCR-F1/R1 and PCR-F2/R2, respectively. The six ASPE primers used to detect the WT, G1, or G2 alleles are illustrated with directional arrows and labeled as c.1072WT, c.1072A>G, c.1200WT, c.1200T>G, c.1212_1217WT, and c.1212_1217del6. Primer sequences are listed in Materials and Methods and Table 1.