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HAI-1 can be expressed recombinantly in MDCK cells. Cell extracts of transfected (T) and non-transfected (NT) MDCK cells were analyzed by western blotting. Positions of marker proteins and their molecular masses in kDa are indicated. After stripping, the blots were reprobed with an antibody against the transferrin receptor (TR) as a loading control, indicating that equal amounts of cell extracts were applied to each lane.
