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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biochem J. 2008 Jul 15;413(2):251–259. doi: 10.1042/BJ20071496

Figure 2.

Figure 2

HAI-1 is mainly located on the basolateral plasma membrane together with its cognate protease matriptase. MDCK cells expressing HAI-1 were labelled for HAI-1 in the absence of detergent to detect HAI-1 at the plasma membrane (HAI-1surface)(a and d) thereafter labelling for HAI-1 was repeated using the same primary antibody but a different secondary antibody in the presence of detergent to detect both intracellular and extracellular HAI-1 (HAI-1total) (b and e). Overlay of a and b are shown in c, and overlay of d and e are shown in f. Tight filter grown MDCK cells were biotinylated from either the apical side (g) or the basolateral side (j) and thereafter labelled for HAI-1 (h and k). Overlay of g and h is shown in i and overlay of j and k is shown in l. HAI-1 is mainly located on the basolateral membrane in the mouse mammary gland. Mouse mammary gland from mouse at 10th day of lactation was labelled for HAI-1 and analyzed by confocal microscopy (m). MDCK cells expressing HAI-1 were transiently transfected with an expression vector coding for matriptase. Cells were labelled for HAI-1 (n) and matriptase (o). Overlay of n and o is shown in p.