Figure 3.

Gnrhr mRNA expression levels in the pituitary glands of prepubertal KERαKO mice are elevated relative to prepubertal WT mice. Relative Gnrhr mRNA expression levels for WT and KERαKO mice that were left intact (sham) on P14 or P34 and then euthanized 2 days later (P16 or P36, respectively). Gnrhr mRNA expression levels are normalized to Rn18s and compared relative to expression levels in P16 WT mice. Two-way ANOVA analysis indicated no significant interaction between genotype and age (P = .10). Age significantly affected Gnrhr mRNA expression independent of genotype (P < .05). *, P < .05; **, P < .01 as determined by post hoc comparisons. All data represented as mean ± SEM.