Figure 4.

E₂ suppresses Kiss1 mRNA expression in prepubertal WT mice but has no effect on Kiss1 mRNA expression in prepubertal KERαKO mice. Relative Kiss1 mRNA expression levels for WT and KERαKO mice that were left intact (sham) or bilaterally OVX and implanted with a capsule containing vehicle (V) or E₂ on P14 and then euthanized 2 days later (P16). Kiss1 mRNA expression levels are normalized to Rn18s and compared relative to expression levels in sham WT mice. Two-way ANOVA analysis indicated a significant interaction between genotype and treatment (P < .05). Genotype and treatment significantly affected Kiss1 mRNA expression independent of each other (P < .05). *, P < .05; **P, < .01; ***, P < .001 as determined by post hoc comparisons. All data represented as mean ± SEM.