Table 1.
Antibody Table
| Peptide/Protein Target | Antigen Sequence | Name of Antibody | Manufacturer, Catalog # | Species Raised in; Monoclonal or Polyclonal | Dilution |
|---|---|---|---|---|---|
| E-cadherin | Anti-E-cadherin | DB Bioscience, 610 182 | Mouse monoclonal (clone 36) | 0.25 μg/ml | |
| Ki-67 | Anti-Ki-67 | DB Pharmingen, 556 003 | Mouse monoclonal (clone B56) | 2 μg/ml | |
| Nkx2–1 | Anti-Thyroid Transcription Factor-1 | Dako, M3575 | Mouse monoclonal (clone 8G7G3/1) | 1/500 | |
| GFP | Anti-GFP | Cell signaling, 2956 | Rabbit monoclonal | 1/200 | |
| GFP | aa1-246 | Anti-GFP | Abcam, ab6673 | Goat polyclonal | 1/250 |
| ZO-1 | aa463-1109 | Anti-ZO-1 | Invitrogen, 61-7300 | Rabbit polyclonal | 2.5 μg/ml |
| Laminin | Anti-laminin | Sigma, L9393 | Rabbit polyclonal | 5 μg/ml | |
| F4/80 | Anti-F4/80 | Abcam, ab6640 | Rat monoclonal (clone CI:A3-1) | 3.4 μg/ml | |
| F4/80 | Anti-F4/80 | eBioscience, 14-4801 | Rat monoclonal (clone BM8) | 5 μg/ml | |
| MHCII | Anti-MHC class II (I-A/I-E) | Millipore, MABF33 | Rat monoclonal (clone M5/114) | 10 μg/ml | |
| CD11b | Anti-CD11b | Biolegend, 101 202 | Rat monoclonal (clone M1/70) | 5 μg/ml | |
| CD11c | Anti-CD11c | Abcam, ab11029 | Rat monoclonal (clone 3.9) | 10 μg/ml | |
| CD45 | Anti-CD45 | Biolegend, 103 102 | Rat monoclonal (clone 30-F11) | 5 μg/ml | |
| Ly6C | Anti-Ly6C | Thermo Scientific, MA1-81 899 | Rat monoclonal (ER-MP20) | 10 μg/ml | |
| KDEL | Anti-KDEL | Abcam, ab50601 | Rat monoclonal (MAC 256) | 1/300 | |
| LAMP-1 | Anti-LAMP-1 1D4B | Hybridoma Bank, #1D4B | Rat monoclonal (clone 1D4B) | 1/75 |