Figure 2.

Distinct hepatic target gene expression patterns after acute and chronic T₃. Adult male mice (8–10 wk old) were treated with 20 μg liothyronine (L-T₃) per 100 g body weight for 14 days. Livers were harvested from mice 6 hours after the first L-T₃ injection (acute T₃) as well as after the last L-T₃ injection (chronic T₃). A and B, Venn diagrams showing differentially expressed genes at indicated T₃-treatment periods in positively (A) and negatively (B) regulated genes from microarray data as described in Materials and Methods. C, Representative heat map displaying the top 50 up-regulated genes after acute T₃ and/or chronic T₃, compared with nontreated mice. Genes underlined show genes induced in common with both exposure protocols. Genes in bold show genes analyzed by RT-qPCR. Row-normalized gene expression levels are represented according to the colored bar scale at the bottom of the figure (n = 3/group).