Figure 6.

Distinct temporal patterns of histone modifications induced by T₃ on Dio1, Thrsp, and Cyp17a1 gene promoters. Adult male mice (8–10 wk old) were treated with 20 μg liothyronine (L-T₃) per 100 g body weight for 14 days. A–C, Histone modifications in the promoters of these three target genes induced by T₃. ChIP-qPCR analyses were performed with anti-H3K9/K14ac or anti-H3K4me3 at indicated T₃-treatment periods after the first and the last L-T₃ injections. No treatment (blank); acute T₃ treatment (black); chronic T₃ treatment (hashed); early T₃ withdrawal (hashed); and T₃ withdrawal (striped) are shown. Note that the same data points were used for the chronic T₃ treatment and early T₃ withdrawal groups (hashed). Parentheses represent primer location referring to the transcription start site of reference sequencing. Data are presented as means ± SEM (n = 4–5/group). *, P < .05, **, P < .01, ***, P < .001, compared with nontreated mice (0 d) by one-way ANOVA.