Table 1.
Primers Used in Generating and Genotyping Transgenic Mice
| Prop1 Homology Domain | cre Cassette Homology Domain | |
|---|---|---|
| Prop1-cre transgene construction | ||
| Exon 2 A | GTGAGGATGGGCTGGTGGTCCCATTAATAAGGGGGTCTCCCTCCGTTTTTCTCCCTCCATAGACAGGAGCTCTGAG | ATGGTGCCCAAGAAGAAGAGGAAA |
| Exon 2 B | GGCGGCGCCGGGAGTGGGGACGGCCTCTCTGTGGGCAAAGCTTAGGTCTCCCCAGCTCTGTACCAGAGAGCCTCCTATAA | CTATACGAAGTTATAAGCTT |
| Prop1-cre transgene genotyping | ||
| A | CTGCACACAGACAGGAGCAT | |
| B | GGTCTCCCTCCGTTTTTCTC | |
| 25-kb Prop1 BAC transgene genotyping | ||
| A | AATTAACCCTCACTAAAGGG | |
| B | AAGGGCAGGTAAGATGCTGA | |
| 10-kb Prop1 BAC transgene genotyping | ||
| A | AAACCCCCAGAGAAGAGGAA | |
| B | GACCATGATTACGCCAAGCTA | |
| HsPROP1 BAC transgene genotyping | ||
| A | GGAGGGAACAGGTGTGGAG | |
| B | GGTGTCTCTCAGGCAACAGG | |
Each primer is listed 5′–3′, and A and B indicate forward and reverse primers, respectively.