Figure 9.

Comparing the PMF profiles of the WT (black) and mutant (green) α-actinins shows that a new minimum (at 3.9 nm) was created upon the K237E mutation. The CH1-CH2 distance was taken as the reaction coordinate for the umbrella sampling calculations. Profiles are fairly similar up to 3 nm but deviate after that. The slope of the mutant PMF is slightly higher but reaches a short plateau around 3.3 nm. The error bars were calculated using the bootstrap analysis. To see this figure in color, go online.